Molecular Diagnostics : A Practical Manual

Description

Molecular Diagnostics : A Practical Manual By Diwakar Singh, K.P. Suthar, Reetu Mehta, CONTENTS :

1. Diagnosis of Drought Tolerance in Crop Plants by PEG Method
2. Identification of Salt Tolerant Crop Variety by Glycine Betaine (GB) Method
3. Total Protein Estimation by Folin-Lowry’s Method
4. Protein Electrophoresis Using SDS-PAGE
5. Enzyme Extraction and Assay Study of Polyphenol Oxidase (PPO; EC 1.14.18.1)
6. Enzyme Extraction and Assay Study of Catalase (EC 1.11.1.6)
7. Enzyme Extraction and Assay Study of Guaiacol Peroxidase (POX) (EC 1.11.1.7)
8. Enzyme Extraction and Activity Study of Superoxide Dismutase (EC 1.15.1.1)
9. Peroxidase Isozyme Study by Electrophoresis Method
10. DNA Isolation from Plant Sample and Quantification by Spectrophotometer
11. Quality Check of Plant Genomic DNA on Agarose Gel Electrophoresis
12. Randomly Amplified Polymorphic DNA (RAPD) and Electrophoresis
13. Southern Blotting Experiment
14. Total RNA Isolation from Plants and its Quantification by Spectrophotometer
15. Quality Check of Total RNA on Agarose Gel
16. cDNA Synthesis from Isolated Plant Total RNA
17. Indirect Competitive ELISA
18. Objective Questions