Techniques in Molecular Diagnostics

Description

Techniques in Molecular Diagnostics By Kandarpa Kumar Saikia, CONTENTS : 1. Introduction to Molecular Diagnostics by Partha Pratim Sarma 2. General Calculation for Making Solutions in Molecular Diagnostic Laboratory by Pranjal Kr. Baruah 3. Basic Requirements for Setting-up a Molecular Diagnostic Laboratory by Kandarpa Kr. Saikia 4. Nucleic Acid Extraction Protocols by Partha Pratim Sarma and Kandarpa Kr. Saikia 5. Protocol to Perform Agarose Gel Electrophoresis by Sukanta Nath and Partha Pratim Sarma 6. Protocol for Qualitative PCR Using Genomic DNA or cdna as Template by Kandarpa Kr. Saikia and Sukanta Nath 7. DNA Sequence Analysis by Kandarpa Kr. Saikia and Vedant Vikrom Borah 8. Protocol for Synthesis of cDNA from Total RNA by Sukanta Nath and Partha Pratim Sarma 9. Protocol to Perform RT-PCR (Reverse Transcriptase PCR for Gene Expression Studies) by Kandarpa Kr. Saikia 10. Protocol to Perform Quantitative Real Time PCR (qpcr) by Partha Pratim Sarma and Sukanta Nath 11. Protocol to Perform FISH by Manoj Kr. Panigrahi 12. Examples of Biomarker Detection Protocols Using Molecular Diagnostic Tools -Acute Myeloid Leukaemia, Glioma and Chronic Myeloid Leukaemia Molecular Markers by Kandarpa Kr. Saikia, Partha Pratim Sarma, Sukanta Nath and Dushyant Kumar 13. Quality Assurance and Quality Control in Molecular Diagnostics by Rupjyoti Kalita and Kandarpa Kr. Saikia